4 edition of Phosphopeptide mapping of axon guidance molecules by Nano-ESI tandem mass spectrometry found in the catalog.
Phosphopeptide mapping of axon guidance molecules by Nano-ESI tandem mass spectrometry
Kathleen Leslie Binns
Thesis (M.Sc.) -- University of Toronto, 2002.
|Series||Canadian theses = -- Th`eses canadiennes|
|The Physical Object|
|Pagination||2 microfiches : negative.|
Families of axon guidance molecules Recently, substantial progress has been made in the iso- Semaphorins Semaphorins constitute a family of secreted and mem- brane proteins with representatives identified in both in- sects and vertebrates. Their distinctive feature is the Phosphopeptide Array Platform for Cellular Signaling Network Protein Profiling in Breast Cancer Cells. Srinivasan Krishnamoorthy 1, Ailing Hong 2, Zhonghua Liu 1, Ruijuan Zhu 1, Xin Wang 1, Yulu Zhang 1, Qi Zhu 2, Xiaochuan Zhou 2, Tongbin Li 2, Xiaolian Gao 1 1 Department of Biology and Biochemistry, University of Houston, Houston, TX , USA. 2 LC Sciences, Houston, TX
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Protein phosphatase 2A (PP2A) is indispensable in development, and deficits of PP2A and deterioration of neuronal axons have been observed in several neurodegenerative disorders, but the direct link between PP2A and the neuronal axon development is still missing. Here, we show that PP2A is essential for axon development in transfected rat brain and the dissociated hippocampal neurons. During axon guidance, it is presently thought that: i), external signals (e.g., netrin) are integrated into the intracellular signaling cascade via membrane receptors; ii), these signals interact with GTPases (Cdc42, Rac, and RhoA), which also cross talk with each other; and iii), the resultant signals from the GTPases regulate cytoskeleton dynamics.
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A multidimensional electrospray MS-based approach to phosphopeptide mapping. (III) affinity chromatography and matrix-assisted laser desorption/ionization tandem mass spectrometry for recovery, detection and sequencing of phosphopeptides.
Rapid Commun Mass Cited by: 7. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates. Methods Enzymol. Gould, K. Author: Jill Meisenhelder, Peter van der Geer.
Phosphopeptide structure analysis by tandem mass spectrometry is usually done with positively charged ions. 10 However, cleavage of the phosphate group is often the dominant process when collisional activation or infra-red multi-photon dissociation activation methods are employed.
11, 12 Alternatively, electron capture dissociation (ECD) and Cited by: The central component in the road trip of axon guidance is the growth cone, a dynamic structure that is located at the tip of the growing axon. The phosphopeptide map analysis revealed the.
Phosphopeptide mapping of these phosphorylated residues allows investigation into the positive and negative regulatory roles these sites may play in vivo. In addition, phosphopeptide mapping can uncover the specific phosphorylated residue and, hence, kinase recognition sites, thus helping in the identification of the relevant kinase(s) and/or Cited by: 6.
Chemorepulsive Axon Guidance Molecules. Several axon guidance molecules are expressed in the adult CNS, where they are implicated in network stabilization by limiting neuronal growth, and their expression is regulated following injury. 27 Among them, semaphorins, a large family of secreted and membrane-associated proteins involved in axon.
Guidance Cues and Their Receptors. Netrins. The discovery of netrins came as the remarkable convergence of the search for a chemoattractant for vertebrate commissural axons (3, 4), and the analysis of genes required for circumferential axon guidance inCaenorhabditis elegans (5, 6).Across more than million years of evolution, netrins have retained the function of attracting axons.
Abstract. Caenorhabditis elegans UNC-5 and its mammalian homologues such as RCM are receptors for the secreted axon guidance cue UNC-6/netrin and are required to mediate the repulsive effects of UNC-6/netrin on growth cones. We find that C. elegans UNC-5 and mouse RCM are phosphorylated on tyrosinein vivo.
elegans UNC-5 tyrosine phosphorylation is reduced in unc-6 null mutants, and RCM. Axon guidance represents a key stage in the formation of neuronal network.
Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are read by growth cone receptors, and signal transduction pathways downstream of these receptors converge onto the Rho GTPases to elicit changes in.
Science 15 Nov Vol. Issuepp. DOI: /science lucidum. Furthermore, the classical axon guidance molecules ephrin (8), semaphorin (9, 10), and netrin (11) are also involved in the pruning and stratum lucidum-speciﬁc targeting of the mossy ﬁbers.
Although several axon guidance cues act together to regulate the mossy ﬁber projections, under some epileptic conditions. Ta ble 2: Phosphotyrosine-containing proteins iden ti ed with 2DGE-based Western blotting and tandem mass spectrometry Spot numbe r Swiss-Prot No.
Protein name Monoisotopic mass of neutral peptide. PHOSPHOPROTEOMICS Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry Benjamin L.
Parker,1,2* Guang Yang,1,2* Sean J. Humphrey,3 Rima Chaudhuri,1,2 Xiuquan Ma,1,2 Scott Peterman,4 David E. James1,2,5† A major goal in signaling biology is the establishment of high-throughput quantitative methods for. Analytical methods such as high-performance liquid chromatography (HPLC) with fluorescent detectors [12,13,14] and high-performance liquid chromatography coupled with mass spectrometric detectors [15,16] have been developed for quantitative detection of AFM these procedures rely on expensive instruments, qualified staff and complicated time-consuming pretreatments.
Table 1. Amino acid sequences and the mass to charge ratios of the four synthetic phosphopeptides (MassPREP™ Phosphopeptide Standards PN ). Figure 1.
ESI/MS spectrum of the Waters MassPREP™ phosphopeptide standards. The doubly protonated phosphopeptide ions are labeled. Ying Qing Yu, Iain Campuzano and Martin Gilar.
Nano-ESI-MS/MS-based phosphopeptide mapping. Purified EphA4 CYTO was autophosphorylated in the presence of 10 mM MgCl 2 –1 mM ATP and trypsin digested to completion at 37°C for 3 h using a ratio of protein to protease.
Phosphopeptides were desalted using ZipTip desalting columns (Millipore) equilibrated in 5% formic acid, washed with. A mass spectrometer consists of an ion source, a mass analyzer that measures the mass-to-charge ratio (m/z) of the ionized analytes, and a.
1 Poster Reprint ASMS Ofﬂine Nano-ESI Phosphopeptide Analysis with Carbon, TiO 2 and ZrO 2 Wall-Coated Trap’nTips™ Christopher J. Toher1, Adam W. Perala1, Mukta M. Shukla2, Ashok K. Shukla2, Gary A. Valaskovic1 1New Objective, Inc., Woburn.
Detection of Phospho-Akt (S) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either untreated (open histogram), or treated with nM Calyculin A (Catalog # ) for 30 min (filled histogram), was stained with Mouse Anti-Human/Mouse Phospho-Akt (S) Alexa Fluor® conjugated Monoclonal Antibody (Catalog # ICG).
An axon's function is dictated by its morphology and, thus, by the properties and organization of the axonal cytoskeleton. Leterrieret al. describe how advances in super-resolution and live-cell.
Neuron Article Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function Jean-Michel Cioni,1 Hovy Ho-Wai Wong,1 Dario Bressan,2 Lay Kodama,1 William A.
Harris,1 and Christine E. Holt1,3,* 1Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK 2Cancer .Netrins are diffusible axon guidance cues most famous for attracting axons to the CNS midline . The ﬂy has two Netrin genes, NetA and NetB, that are required for midline and motor neuron axon guidance [13,14].
The genes appear to be the product of tandem duplication and display a high degree of functional overlap.Traumatic brain injury (TBI), as a neurological injury, becomes a leading cause of disability and mortality due to lacking effective therapy.
About 75% of TBI is mild traumatic brain injury (mTBI). However, the complex molecular mechanisms underlying mTBI pathophysiology remains to be elucidated. In this study, iTRAQ-based quantitative proteomic approach was employed to measure temporal-global.